Cummings School of Veterinary Medicine at Tufts University
M.S. Amarendhra Kumar

Professor

Peptidergic Neurons
Neuroendocrinology

Campus Phone:
508-839-7967

Fax:
508-839-7969

Education

  • PhD - University of Florida - 1980
  • MS - Kansas State University - 1976
  • B.V.Sc and M.V.Sc - Mysore Veterinary College, India - 1970 and 1973

Reduction and Fixation of Cranial Cervical Fracture/Luxations using Screws and Polymethylmethacrylate (PMMA) Cement: A Distraction Technique Applied to the Base of the Skull in Thirteen Dogs

Author(s):Pike, F.S. | Kumar, M.S.A. | Boudrieau, R.J.
Publication year: 2012
Journal / Book title: Veterinary Surgery

Nasopharyngeal turbinates in brachycephalic dogs and cats

Author(s):Ginn, J.A. | Kumar, M.S.A. | McKiernan, B.C. | Powers, B.E.
Publication year: 2008
Journal / Book title: Journal of the American Animal Hospital Association

Predicting the onset of parturition in the goat by determining progesterone levels by enzyme immunoassay

Author(s):Singer, L.A. | Kumar, M.S.A. | Gavin, W. | Ayres, S.L.
Publication year: 2004
Journal / Book title: Small Ruminant Research

Lead toxicosis in cats - A review

Author(s):Knight, T.E. | Kumar, M.S.A.
Publication year: 2003
Journal / Book title: Journal of Feline Medicine and Surgery

Effect of nitrous oxide on intracellular events of GT1-7 GnRH-secreting neurons.

Author(s):Zhang, Q. | Rosenberg, M. | Kugel, G. | Agarwal, R.K. | Phillips, J. | Kumar, M.S.
Publication year: 2003
Journal / Book title: Anesthesia progress

Signaling by bone morphogenetic proteins and Smad1 modulates the postnatal differentiation of cerebellar cells

Author(s):Angley, C. | Kumar, M. | Dinsio, K.J. | Hall, A.K. | Siegel, R.E.
Publication year: 2003
Journal / Book title: Journal of Neuroscience

Antisense suppression of GABAA receptor β subunit levels in cultured cerebellar granule neurons demonstrates their importance in receptor expression

Author(s):Kumar, M. | Luo, X. | Quirk, P.L. | Siegel, R.E.
Publication year: 2001
Journal / Book title: Journal of Neurochemistry

Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses

Author(s):Kirker-Head, C.A. | Chandna, V.K. | Agarwal, R.K. | Morris, E.A. | Tidwell, A. | O'Callaghan, M.W. | Rand, W. | Kumar, M.S.A.
Publication year: 2000
Journal / Book title: American Journal of Veterinary Research

Regional distribution of gonadotropin-releasing hormone-like, β-endorphin-like, and methionine-enkephalin-like immunoreactivities in the central nervous system of the goat

Author(s):Karuri, A.R. | Ayres, S. | Kumar, M.S.A.
Publication year: 2000
Journal / Book title: Brain Research Bulletin

Effects of halothane and methoxyflurane on the hypothalamic-pituitary- adrenal axis in rat

Author(s):Karuri, A.R. | Engelking, L.R. | Kumar, M.S.A.
Publication year: 1998
Journal / Book title: Brain Research Bulletin

Alterations in catecholamine turnover in specific regions of the rat brain following acute exposure to nitrous oxide

Author(s):Karuri, A.R. | Kugel, G. | Engelking, L.R. | Kumar, M.S.A.
Publication year: 1998
Journal / Book title: Brain Research Bulletin

Effects of halothane and methoxyflurane on regional brain and spinal cord substance P-like and betaendorphin-like immunoreactivities in the rat

Author(s):Karuri, A.R. | Agarwal, R.K. | Engelking, L.R. | Kumar, M.S.A.
Publication year: 1998
Journal / Book title: Brain Research Bulletin

Effect of low and high doses of nitrous oxide on preproenkephalin mRNA and its peptide methionine enkephalin levels in the hypothalamus

Author(s):Agarwal, R.K. | Kugel, G. | Karuri, A. | Gwosdow, A.R. | Kumar, M.S.A.
Publication year: 1996
Journal / Book title: Brain Research

Chronic vaginal prolapse during pregnancy in a bitch.

Author(s):Memon, M.A. | Pavletic, M.M. | Kumar, M.S.
Publication year: 1993
Journal / Book title: Journal of the American Veterinary Medical Association

Effect of midazolam infusion and flumazenil administration on epinephrine arrhythmogenicity in dogs anesthetized with halothane

Author(s):Court, M.H. | Dodman, N.H. | Greenblatt, D.J. | Agarwal, R.K. | Kumar, M.S.A.
Publication year: 1993
Journal / Book title: Anesthesiology

Distribution of catecholamines in the central nervous system of the pig

Author(s):Agarwal, R.K. | Chandna, V.K. | Engelking, L.R. | Lightbown, K. | Kumar, M.S.A.
Publication year: 1993
Journal / Book title: Brain Research Bulletin

Interleukin-1-induced corticosterone release occurs by an adrenergic mechanism from rat adrenal gland

Author(s):Gwosdow, A.R. | O'Connell, N.A. | Spencer, J.A. | Kumar, M.S.A. | Agarwal, R.K. | Bode, H.H. | Abou-Samra, A.B.
Publication year: 1992
Journal / Book title: American Journal of Physiology - Endocrinology and Metabolism

Distribution of substance P, GnRH, Met-enkephalin in the central nervous system of the pig

Author(s):Kumar, M.S.A. | Becker, T. | Ebert, K.
Publication year: 1991
Journal / Book title: Brain Research Bulletin

Disulfiram administration affects substance P-like immunoreactive and monoaminergic neural systems in rodent brain

Author(s):Marchand, J.E. | Hershman, K. | Kumar, M.S.A. | Thompson, M.L. | Kream, R.M.
Publication year: 1990
Journal / Book title: Journal of Biological Chemistry

Interleukin 1 stimulation of the hypothalamic-pituitary-adrenal axis

Author(s):Gwosdow, A.R. | Kumar, M.S.A. | Bode, H.H.
Publication year: 1990
Journal / Book title: American Journal of Physiology - Endocrinology and Metabolism

General Research Interests

Central Integrative Processes Controlling Gonadal Function: Role of peptidergic neurons, with emphasis on GnRH-Catecholamine-endogenous Opioid peptidergic neural interaction

Selected Research Projects

  • Reproductive hazards associated with exposure to nitrous oxide: Epidemiological data from human and animal studies indicate that chronic exposure to nitrous oxide (N2O) results in impaired reproductive function and decreased fertility rates. Preliminary studies performed in our laboratory indicate that N2O exposure disrupts estrous cyclicity with concomitant alterations in diencephalic opioid peptide concentrations, catecholamine turnover rates, and gonadotropin-releasing hormone (GnRH) concentrations. Furthermore, in vitro studies performed in our laboratory, utilizing immortalized GnRH neurons (GT1-7 cells), indicate that exposure to N2O results in significant decreases in proGnRH mRNA concentrations. These results are consistent with our overall hypothesis that N2O impairs hypothalamic control of gonadotropin release, either by directly acting on GnRH neurons or by altering upstream neural influences on GnRH neurons.

  • Effects of lead exposure during early development on neural circuits controlling puberty onset and hypothalamic-pituitary axis: This is a collaborative research effort with Drs. Dees and Bratton at Texas A&M University. We will examine the effects of LEAD on the molecular and cellular events upstream of the GnRH neuron crucial to gonadotropin secretion regulation, as well as signaling pathways, and metabolism of GnRH peptide in cultured GT1-7 cells.

Major Specialized Equipment

The laboratory has HPLC setup with UV, on-line radioisotope and electrochemical detection capabilities. Other facilities in the laboratory can support protein work. Other equipment can support histological, immunohistochemical studies on organs/ tissues. The lab also has equipment for conducting routine protein biochemistry, as well as radioimmunoassays.Laboratory Personnel
  • Quanshun Zhang, Post-doctoral fellow