Cummings School of Veterinary Medicine at Tufts University
M. Sawkat Anwer

Distinguished Professor
Associate Dean for Research

Liver Disease
Molecular Physiology and Pharmacology

Campus Phone:
508-839-8788

Fax:
508-839-8787

Education

  • DMVH - Munich University - 1980
  • PhD - Kansas State University - 1973
  • MS - Dhaka University - 1968

Sodium-dependent bile salt transporters of the SLC10A transporter family: More than solute transporters

Author(s):Anwer, M.S. | Stieger, B.
Publication year: 2014
Journal / Book title: Pflugers Archiv European Journal of Physiology

Role of protein kinase C isoforms in bile formation and cholestasis

Author(s):Anwer, M.S.
Publication year: 2014
Journal / Book title: Hepatology

Rab11, but not Rab4, facilitates cyclic AMP- and tauroursodeoxycholate-induced MRP2 translocation to the plasma membrane

Author(s):Won Park, S. | Schonhoff, C.M. | Anwer, M.S. | Webster, C.R.L.
Publication year: 2014
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

Cysteine 96 of Ntcp is responsible for NO-mediated inhibition of taurocholate uptake

Author(s):Ramasamy, U. | Anwer, M.S. | Schonhoff, C.M.
Publication year: 2013
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

Taurolithocholate-induced MRP2 retrieval involves MARCKS phosphorylation by protein kinase Cε{lunate} in HUH-NTCP Cells

Author(s):Schonhoff, C.M. | Webster, C.R.L. | Anwer, M.S.
Publication year: 2013
Journal / Book title: Hepatology

Western blot patterns of serum autoantibodies against optic nerve antigens in dogs with goniodysgenesis-related glaucoma

Author(s):Pumphrey, S.A. | Pizzirani, S. | Pirie, C.G. | Anwer, M.S. | Logvinenko, T.
Publication year: 2013
Journal / Book title: American Journal of Veterinary Research

Intracellular signaling by bile acids

Author(s):Anwer, M.S.
Publication year: 2012
Journal / Book title: Journal of Bio-Science

cAMP-guanine exchange factor protection from bile acid-induced hepatocyte apoptosis involves glycogen synthase kinase regulation of c-jun NH 2-terminal kinase

Author(s):Johnston, A. | Ponzetti, K. | Anwer, M.S. | Webster, C.R.L.
Publication year: 2011
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

Nitric oxide-mediated inhibition of taurocholate uptake involves S-nitrosylation of NTCP

Author(s):Schonhoff, C.M. | Ramasamy, U. | Anwer, M.S.
Publication year: 2011
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

Phosphatidylinositol-3-kinase p110γ contributes to bile salt-induced apoptosis in primary rat hepatocytes and human hepatoma cells

Author(s):Hohenester, S. | Gates, A. | Wimmer, R. | Beuers, U. | Anwer, M.S. | Rust, C. | Webster, C.R.L.
Publication year: 2010
Journal / Book title: Journal of Hepatology

Cyclic AMP stimulates Mrp2 translocation by activating p38α MAPK in hepatic cells

Author(s):Schonhoff, C.M. | Webster, C.R.L. | Anwer, M.S.
Publication year: 2010
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

PKCε-dependent and -independent effects of taurolithocholate on PI3K/PKB pathway and taurocholate uptake in HuH-NTCP cell line

Author(s):Schonhoff, C.M. | Yamazaki, A. | Hohenester, S. | Webster, C.R.L. | Bouscarel, B. | Anwer, M.S.
Publication year: 2009
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

cAMP-GEF cytoprotection by Src tyrosine kinase activation of phosphoinositide-3-kinase p110 β/α in rat hepatocytes

Author(s):Gates, A. | Hohenester, S. | Anwer, M.S. | Webster, C.R.L.
Publication year: 2009
Journal / Book title: American Journal of Physiology - Gastrointestinal and Liver Physiology

Rab4 facilitates cyclic adenosine monophosphate-stimulated bile acid uptake and Na+-taurocholate cotransporting polypeptide translocation

Author(s):Schonhoff, C.M. | Thankey, K. | Webster, C.R.L. | Wakabayashi, Y. | Wolkoff, A.W. | Anwer, M.S.
Publication year: 2008
Journal / Book title: Hepatology

Protein kinase Cδ mediates cyclic adenosine monophosphate-stimulated translocation of sodium taurocholate cotransporting polypeptide and multidrug resistant associated protein 2 in rat hepatocytes

Author(s):Schonhoff, C.M. | Gillin, H. | Webster, C.R.L. | Anwer, M.S.
Publication year: 2008
Journal / Book title: Hepatology

PKCζ is required for microtubule-based motility of vesicles containing the ntcp transporter

Author(s):Sarkar, S. | Bananis, E. | Nath, S. | Anwer, M.S. | Wolkoff, A.W. | Murray, J.W.
Publication year: 2006
Journal / Book title: Traffic

Dephosphorylation of Ser-226 facilitates plasma membrane retention of Ntcp

Author(s):Anwer, M.S. | Gillin, H. | Mukhopadhyay, S. | Balasubramaniyan, N. | Suchy, F.J. | Ananthanarayanan, M.
Publication year: 2005
Journal / Book title: Journal of Biological Chemistry

4. MECHANISMS OF BILE FORMATION AND CHOLESTASIS

Author(s):Anwer, M.S.
Publication year: 2004
Journal / Book title: Principles of Medical Biology

Cross-talk between protein kinases Cζ and B in cyclic AMP-mediated sodium taurocholate co-transporting polypeptide translocation in hepatocytes

Author(s):McConkey, M. | Gillin, H. | Webster, C.R.L. | Anwer, M.S.
Publication year: 2004
Journal / Book title: Journal of Biological Chemistry

Cellular Regulation of Hepatic Bile Acid Transport in Health and Cholestasis

Author(s):Anwer, M.S.
Publication year: 2004
Journal / Book title: Hepatology

General Research Interests

Functional abnormalities associated with various liver diseases lead to accumulation of toxic products (such as bilirubin in jaundice and bile acids) in blood and premature death of liver cells. One of the major determinants is the inability of the liver to properly regulate bile acid transport from blood to bile. Studies are conducted to better understand the mechanisms regulating bile acid transport. Specifically, studies are designed to determine the role of various kinases and phosphatases in the regulation of bile acid transport using primary hepatocytes, hepatic cell lines and transgenic mice. Techniques used are cell culture, transfection of cell lines, solute uptake, western blots, protein phosphorylation, Protein S-nitrosylation, etc.

Selected Research Projects

  • Role of protein kinase Cs in the regulation of hepatic transporters.
  • Mechanism of taurolithocholate-induced cholestasis.
  • Role of protein S-nitrosylation in sepsis-induced cholestasis.
  • Mechanisms of anticholestatic effects of cAMP and tauroursodeoxycholate.

Research Interests by Area

Hepatic Diseases
  • Mechanism of bile formation and cholestasis
Training Grants
  • NIH Short term research training for DVM students
  • Veterinary Research Manpower Development (US Army Medical Command)

Major Specialized Equipment

  • Fluorescent spectrophotometers
  • UV-VIS spectrophotometers
  • Scintillation Counter
  • Gel-Electrophoresis (1D & 2D) apparatus
  • Film developer
  • Cell culture facility
  • Fluroescence microscope

Lab Personnel

  • Holly Jameson, Sr. Research Coordinator
  • Ariel Hobson, Research Technician
  • Umadevi Ramasamy, Research Technician
  • Sewon Park, Graduate Student
  • Christopher Schonhoff, Ph.D., Research Assistant Professor