Cummings School of Veterinary Medicine at Tufts University
M. Sawkat Anwer

Distinguished Professor
Associate Dean for Research

Liver Disease
Molecular Physiology and Pharmacology

Campus Phone:
508-839-8788

Fax:
508-839-8787

Education

  • DMVH - Munich University - 1980
  • PhD - Kansas State University - 1973
  • MS - Dhaka University - 1968
  1. Ponzetti, K., King, M., Gates, A., Anwer, M.S., Webster, C.R.L.: Cyclic AMP-guanine exchange factor activation inhibits JNK-dependent lipopolysaccharide-induced apoptosis in rat hepatocytes. Hepatic Medicine: Evidence and Research 2: 1-11, 2010. PMCID:3131672
  2. Schonhoff, C. M, Webster, C.R.L., Anwer, M.S.: Cyclic AMP stimulates MRP2 translocation by activating p38a MAPK in hepatic cells. Am J Physiol Gastrointest Liver Physiol. 298:G667-G674, 2010. PMCID: 20203059
  3. Hohenester, S., Gates, A., Wimmer, R., Beuers, U., Anwer, M.S., Rust, C., Webster, C.R.L.: Phosphatidylinositol-3-kinase p110γ contributes to bile salt-induced apoptosis in primary rat hepatocytes and human hepatoma cells. J. Hepatol. 53: 918-926, 2010. PMCID: 20675006
  4. Schonhoff, C.M., Ramasamy, U, Anwer, M.S.: Nitric Oxide-Mediated Inhibition of Taurocholate Uptake involves S-Nitrosylation of NTCP. Am. J. Physiol. 300:G364-G370, 2011. PMCID: 21109590
  5. Johnston, A., Roberts, K., Anwer, M.S., Webster, C.R.L.: cAMP-guanine exchange factor protection from bile acid induced hepatocyte apoptosis involves glycogen synthase kinase regulation of C-Jun-NH terminal kinase. Am. J. Physiol. 301: G385-G400, 2011. PMCID:

General Research Interests

Functional abnormalities associated with various liver diseases lead to accumulation of toxic products (such as bilirubin in jaundice and bile acids) in blood and premature death of liver cells. One of the major determinants is the inability of the liver to properly regulate bile acid transport from blood to bile. Studies are conducted to better understand the mechanisms regulating bile acid transport. Specifically, studies are designed to determine the role of various kinases and phosphatases in the regulation of bile acid transport using primary hepatocytes, hepatic cell lines and transgenic mice. Techniques used are cell culture, transfection of cell lines, solute uptake, western blots, protein phosphorylation, Protein S-nitrosylation, etc.

Selected Research Projects

  • Role of protein kinase Cs in the regulation of hepatic transporters.
  • Mechanism of taurolithocholate-induced cholestasis.
  • Role of protein S-nitrosylation in sepsis-induced cholestasis.
  • Mechanisms of anticholestatic effects of cAMP and tauroursodeoxycholate.

Research Interests by Area

Hepatic Diseases
  • Mechanism of bile formation and cholestasis
Training Grants
  • NIH Short term research training for DVM students
  • Veterinary Research Manpower Development (US Army Medical Command)

Major Specialized Equipment

  • Fluorescent spectrophotometers
  • UV-VIS spectrophotometers
  • Scintillation Counter
  • Gel-Electrophoresis (1D & 2D) apparatus
  • Film developer
  • Cell culture facility
  • Fluroescence microscope

Lab Personnel

  • Holly Jameson, Sr. Research Coordinator
  • Ariel Hobson, Research Technician
  • Umadevi Ramasamy, Research Technician
  • Sewon Park, Graduate Student
  • Christopher Schonhoff, Ph.D., Research Assistant Professor