Jean Mukherjee - DVM, PhD, DACVM

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Assistant Professor
Microbiology, Parasitology, Animal Models, Antibody-Based Therapeutics
Department of Public Health and Community Medicine, Tufts School of Medicine

Campus Phone:
508-887-4756
x84756

I am a faculty member at Tufts Veterinary School with a dual background in veterinary medicine and infectious diseases.  I have extensive experience with animal model development, in vivo evaluation of candidate therapeutics against infectious and toxin-mediated disease and development of monoclonal antibodies (mAbs).  I currently manage a Select Agent Facility that supports both in vivo and in vitro work with botulinum toxin (BoNT).  My group has been responsible for establishment and conduct of both the mouse lethality and mouse/rat hindlimb paresis assays for multiple BoNT toxinotypes.  We have used these assays extensively for evaluation of candidate biological and small-drug inhibitor molecules.  Other models of infectious or toxin-mediated disease that I have utilized include: Murine models of cryptococcosis (intraperitoneal, intravenous, intrathecal, intratracheal); murine models of Shiga toxin activity; the EHEC gnotobiotic piglet model; the Clostridium difficile hamster model; and the murine thigh infection model for evaluation of candidate therapeutics against bacterial pathogens.

Education

  • PhD - Albert Einstein College of Medicine - 1992 
  • MS - Albert Einstein College of Medicine - 1991
  • BA - Hamline University - 1989

Board Certification

  • Diplomate of the American College of Veterinary Microbiologists (DACVM)

Adenovirus vector expressing Stx1/Stx2-neutralizing agent protects piglets infected with Escherichia coli O157: H7 against fatal systemic intoxication

Author(s):Sheoran, A.S. | Dmitriev, I.P. | Kashentseva, E.A. | Cohen, O. | Mukherjee, J. | Debatis, M. | Shearer, J. | Tremblay, J.M. | Beamer, G. | Curiel, D.T. | Shoemaker, C.B. | Tzipori, S.
Publication year: 2015
Journal / Book title: Infection and Immunity

A single VHH-based toxin-neutralizing agent and an effector antibody protect mice against challenge with Shiga toxins 1 and 2.

Author(s):Tremblay, J.M. | Mukherjee, J. | Leysath, C.E. | Debatis, M. | Ofori, K. | Baldwin, K. | Boucher, C. | Peters, R. | Beamer, G. | Sheoran, A. | Bedenice, D. | Tzipori, S. | Shoemaker, C.B.
Publication year: 2013
Journal / Book title: Infection and immunity

Antibody against TcdB, but not TcdA, prevents development of gastrointestinal and systemic clostridium difficile disease

Author(s):Steele, J. | Mukherjee, J. | Parry, N. | Tzipori, S.
Publication year: 2013
Journal / Book title: Journal of Infectious Diseases

Sheep monoclonal antibodies prevent systemic effects of botulinum neurotoxin A1

Author(s):Mukherjee, J. | McCann, C. | Ofori, K. | Hill, J. | Baldwin, K. | Shoemaker, C.B. | Harrison, P. | Tzipori, S.
Publication year: 2012
Journal / Book title: Toxins

Allele- and Tir-independent functions of intimin in diverse animal infection models

Author(s):Mallick, E.M. | Brady, M.J. | Luperchio, S.A. | Vanguri, V.K. | Magoun, L. | Liu, H. | Sheppard, B.J. | Mukherjee, J. | Donohue-Rolfe, A. | Tzipori, S. | Leong, J.M. | Schauer, D.B.
Publication year: 2012
Journal / Book title: Frontiers in Microbiology

Biodistribution and elimination kinetics of systemic Stx2 by the Stx2A and Stx2B subunit-specific human monoclonal antibodies in mice

Author(s):Sheoran, A. | Jeong, K.-I. | Mukherjee, J. | Wiffin, A. | Singh, P. | Tzipori, S.
Publication year: 2012
Journal / Book title: BMC Immunology

A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model

Author(s):Mukherjee, J. | Tremblay, J.M. | Leysath, C.E. | Ofori, K. | Baldwin, K. | Feng, X. | Bedenice, D. | Webb, R.P. | Wright, P.M. | Smith, L.A. | Tzipori, S. | Shoemaker, C.B.
Publication year: 2012
Journal / Book title: PLoS ONE

Enhanced actin pedestal formation by enterohemorrhagic Escherichia coli O157:H7 adapted to the mammalian host

Author(s):Brady, M.J. | Radhakrishnan, P. | Liu, H. | Magoun, L. | Murphy, K.C. | Mukherjee, J. | Donohue-Rolfe, A. | Tzipori, S. | Leong, J.M.
Publication year: 2011
Journal / Book title: Frontiers in Microbiology

Efficient serum clearance of botulinum neurotoxin achieved using a pool of small antitoxin binding agents

Author(s):Sepulveda, J. | Mukherjee, J. | Tzipori, S. | Simpson, L.L. | Shoemaker, C.B.
Publication year: 2010
Journal / Book title: Infection and Immunity

Serum concentrations of the third component of complement in healthy dogs and dogs with protein-losing nephropathy

Author(s):Acierno, M.J. | Labato, M.A. | Stern, L.C. | Mukherjee, J. | Jakowski, R.M. | Ross, L.A.
Publication year: 2006
Journal / Book title: American Journal of Veterinary Research

Human antibody against Shiga toxin 2 administered to piglets after the onset of diarrhea due to Escherichia coli O157:H7 prevents fatal systemic complications

Author(s):Sheoran, A.S. | Chapman-Bonofiglio, S. | Harvey, B.R. | Mukherjee, J. | Georgiou, G. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2005
Journal / Book title: Infection and Immunity

Production and characterization of protective human antibodies against Shiga toxin 1

Author(s):Mukherjee, J. | Chios, K. | Fishwild, D. | Hudson, D. | O'Donnell, S. | Rich, S.M. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2002
Journal / Book title: Infection and Immunity

Antibody efficacy in murine pulmonary Cryptococcus neoformans infection: A role for nitric oxide

Author(s):Rivera, J. | Mukherjee, J. | Weiss, L.M. | Casadevall, A.
Publication year: 2002
Journal / Book title: Journal of Immunology

Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection

Author(s):Mukherjee, J. | Chios, K. | Fishwild, D. | Hudson, D. | O'Donnell, S. | Rich, S.M. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2002
Journal / Book title: Infection and Immunity

Urease as a virulence factor in experimental cryptococcosis

Author(s):Cox, G.M. | Mukherjee, J. | Cole, G.T. | Casadevall, A. | Perfect, J.R.
Publication year: 2000
Journal / Book title: Infection and Immunity

Molecular and idiotypic analyses of the antibody response to Cryptococcus neoformans glucuronoxylomannan-protein conjugate vaccine in autoimmune and nonautoimmune mice

Author(s):Nussbaum, G. | Anandasabapathy, S. | Mukherjee, J. | Fan, M. | Casadevall, A. | Scharff, M.D.
Publication year: 1999
Journal / Book title: Infection and Immunity

Antibody-based protection of gnotobiotic piglets infected with Escherichia coli O157:H7 against systemic complications associated with Shiga toxin 2

Author(s):Donohue-Rolfe, A. | Kondova, I. | Mukherjee, J. | Chios, K. | Hutto, D. | Tzipori, S.
Publication year: 1999
Journal / Book title: Infection and Immunity

Monoclonal antibodies reveal additional epitopes of serotype D Cryptococcus neoformans capsular glucuronoxylomannan that elicit protective antibodies

Author(s):Mukherjee, J. | Kozel, T.R. | Casadevaii, A.
Publication year: 1998
Journal / Book title: Journal of Immunology

Effect of immune mechanisms on the pharmacokinetics and organ distribution of Cryptococcal polysaccharide

Author(s):Lendvai, N. | Casadevall, A. | Liang, Z. | Goldman, D.L. | Mukherjee, J. | Zuckier, L.
Publication year: 1998
Journal / Book title: Journal of Infectious Diseases

Characterization of a murine monoclonal antibody to Cryptococcus neoformans polysaccharide that is a candidate for human therapeutic studies

Author(s):Casadevall, A. | Cleare, W. | Feldmesser, M. | Glatman-Freedman, A. | Goldman, D.L. | Kozel, T.R. | Lendvai, N. | Mukherjee, J. | Pirofski, L.-A. | Rivera, J. | Rosas, A.L. | Scharff, M.D. | Valadon, P. | Westin, K. | Zhong, Z.
Publication year: 1998
Journal / Book title: Antimicrobial Agents and Chemotherapy

General Research Interests

Development of immune-based methods for diagnosis, therapy, and prevention of infectious and toxin-mediated diseases of human, veterinary and/or zoonotic concern; animal model development; in vivo evaluation of candidate therapeutics; veterinary microbiology and parasitology.

Selected Research Projects

  • Development and characterization of a mouse monoclonal antibody reagent panel for the guinea pig research model community.
  • Adaptation of the mouse neutropenic thigh model assay for evaluation of candidate therapeutics against clinically relevant, highly antibiotic resistant strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Enterobacter aerogenes, Klebsiella pneumoniae.
  • Development of protective sheep monoclonal antibodies against Botulinum Neurotoxin.
  • Development of a mouse monoclonal antibody reagent panel against Botulinum Neurotoxin toxinotypes A1, A2, B, E.
  • Characterization and evaluation of anti-Stx human monoclonal antibodies for the prevention of Hemolytic Uremic Syndrome (HUS).
  • Identification of the structural components that define protective antibodies against Cryptococcus neoformans.

Teaching

Veterinary Medicine Curriculum:

Microbial Pathogenesis

Emerging & Exotic Diseases of Animals & Initial Accreditation Training

General Parasitology

Basic Pharmacology

Immunology

 

Conservation Medicine Curriculum:

Field & Laboratory Techniques

 

Infectious Diseases & Global Health (MS-IDGH) Curriculum:

Infectious Diseases of Humans and Animals I

Microbiology and Immunology Techniques

Infectious Diseases of Humans and Animals II

Research Interests by Area

Infectious Diseases: Prevention and Control
  • Botulism antidotes and anti-toxins

Laboratory Personnel

  • Karen Baldwin, Animal Specialist
  • Courtney Boucher, Research Technician
  • Kwasi Ofori, Research Assistant