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Jean Mukherjee - DVM, PhD, DACVM

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Assistant Professor
Microbiology, Parasitology, Animal Models, Antibody-Based Therapeutics
Department of Public Health and Community Medicine, Tufts School of Medicine

Campus Phone:
508-887-4756
x84756

I am a faculty member at Tufts Veterinary School with a dual background in veterinary medicine and infectious diseases.  I have extensive experience with animal model development, in vivo evaluation of candidate therapeutics against infectious and toxin-mediated disease and development of monoclonal antibodies (mAbs).  I currently manage a Select Agent Facility that supports both in vivo and in vitro work with botulinum toxin (BoNT).  My group has been responsible for establishment and conduct of both the mouse lethality and mouse/rat hindlimb paresis assays for multiple BoNT toxinotypes.  We have used these assays extensively for evaluation of candidate biological and small-drug inhibitor molecules.  Other models of infectious or toxin-mediated disease that I have utilized include: Murine models of cryptococcosis (intraperitoneal, intravenous, intrathecal, intratracheal); murine models of Shiga toxin activity; the EHEC gnotobiotic piglet model; the Clostridium difficile hamster model; and the murine thigh infection model for evaluation of candidate therapeutics against bacterial pathogens.

Education

  • PhD - Albert Einstein College of Medicine - 1992 
  • MS - Albert Einstein College of Medicine - 1991
  • BA - Hamline University - 1989

Board Certification

  • Diplomate of the American College of Veterinary Microbiologists (DACVM)
  1. Rivera, J., Mukherjee, J., Weiss, L.M., Casadevall, A. 2002. Antibody efficacy in murine pulmonary Cryptococcus neoformans infection: A role for nitric oxide. Journal of Immunology.
  2. Cox, G.M., Mukherjee, J., Cole, G.T., Casadevall, A., Perfect, J.R. 2000. Urease as a virulence factor in experimental cryptococcosis. Infection and Immunity.
  3. Nussbaum, G., Anandasabapathy, S., Mukherjee, J., Fan, M., Casadevall, A., Scharff, M.D. 1999. Molecular and idiotypic analyses of the antibody response to Cryptococcus neoformans glucuronoxylomannan-protein conjugate vaccine in autoimmune and nonautoimmune mice. Infection and Immunity.
  4. Mukherjee, J., Kozel, T.R., Casadevaii, A. 1998. Monoclonal antibodies reveal additional epitopes of serotype D Cryptococcus neoformans capsular glucuronoxylomannan that elicit protective antibodies. Journal of Immunology.
  5. Casadevall, A., Cleare, W., Feldmesser, M., Glatman-Freedman, A., Goldman, D.L., Kozel, T.R., Lendvai, N., Mukherjee, J., Pirofski, L.-A., Rivera, J., Rosas, A.L., Scharff, M.D., Valadon, P., Westin, K., Zhong, Z. 1998. Characterization of a murine monoclonal antibody to Cryptococcus neoformans polysaccharide that is a candidate for human therapeutic studies. Antimicrobial Agents and Chemotherapy.
  6. Franzot, S.P., Mukherjee, J., Cherniak, R., Chen, L.-C., Hamdan, J.S., Casadevall, A. 1998. Microevolution of a standard strain of Cryptococcus neoformans resulting in differences in virulence and other phenotypes. Infection and Immunity.
  7. Lendvai, N., Casadevall, A., Liang, Z., Goldman, D.L., Mukherjee, J., Zuckier, L. 1998. Effect of immune mechanisms on the pharmacokinetics and organ distribution of Cryptococcal polysaccharide. Journal of Infectious Diseases.
  8. Lendvai, N., Casadevall, A., Liang, Z., Goldman, D.L., Mukherjee, J., Zuckier, L. 1997. Effect of humoral immune mechanisms on the pharmacokinetics, organ distribution and tissue localization of an immunologically active fungal polysaccharide antigen. Clinical Infectious Diseases.
  9. Feldmesser, M., Mukherjee, J., Casadevall, A. 1996. Combination of 5-flucytosine and capsule-binding monoclonal antibody in the treatment of murine Cryptococcus neoformans infections and in vitro. Journal of Antimicrobial Chemotherapy.
  10. Mukherjee, J., Cleare, W., Casadevall, A. 1995. Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans. Journal of Immunological Methods.
  11. Mukherjee, J., Feldmesser, M., Scharff, M.D., Casadevall, A. 1995. Monoclonal antibodies to Cryptococcus neoformans glucuronoxylomannan enhance fluconazole efficacy. Antimicrobial Agents and Chemotherapy.
  12. Mukherjee, J., Nussbaum, G., Scharff, M.D., Casadevall, A. 1995. Protective and nonprotective monoclonal antibodies to Cryptococcus neoformans originating from one B cell. Journal of Experimental Medicine.
  13. Mukherjee, J., Scharff, M.D., Casadevall, A. 1995. Variable efficacy of passive antibody administration against diverse Cryptococcus neoformans strains. Infection and Immunity.
  14. Mukherjee, J., Zuckier, L.S., Scharff, M.D., Casadevall, A. 1994. Therapeutic efficacy of monoclonal antibodies to Cryptococcus neoformans glucuronoxylomannan alone and in combination with amphotericin B. Antimicrobial Agents and Chemotherapy.
  15. Casadevall, A., Mukherjee, J., Yuan, R., Perfect, J. 1994. Management of injuries caused by cryptococcus neoformans-contaminated needles. Clinical Infectious Diseases.
  16. Mukherjee, J., Scharff, M.D., Casadevall, A. 1994. Cryptococcus neoformans infection can elicit protective antibodies in mice. Canadian Journal of Microbiology.
  17. Mukherjee, S., Lee, S., Mukherjee, J., Scharff, M.D., Casadevall, A. 1994. Monoclonal antibodies to Cryptococcus neoformans capsular polysaccharide modify the course of intravenous infection in mice. Infection and Immunity.
  18. Zebedee, S.L., Koduri, R.K., Mukherjee, J., Mukherjee, S., Lee, S., Sauer, D.F., Scharff, M.D., Casadevall, A. 1994. Mouse-human immunoglobulin G1 chimeric antibodies with activities against Cryptococcus neoformans. Antimicrobial Agents and Chemotherapy.
  19. Mukherjee, J., Pirofski, L.-A., Scharff, M.D., Casadevall, A. 1993. Antibody-mediated protection in mice with lethal intracerebral Cryptococcus neoformans infection. Proceedings of the National Academy of Sciences of the United States of America.
  20. Mukherjee, J., Casadevall, A., Scharff, M.D. 1993. Molecular characterization of the humoral responses to cryptococcus neoformans infection and glucuronoxylomannan-tetanus toxoid conjugate immunization. Journal of Experimental Medicine.

General Research Interests

Development of immune-based methods for diagnosis, therapy, and prevention of infectious and toxin-mediated diseases of human, veterinary and/or zoonotic concern; animal model development; in vivo evaluation of candidate therapeutics; veterinary microbiology and parasitology.

Selected Research Projects

  • Development and characterization of a mouse monoclonal antibody reagent panel for the guinea pig research model community.
  • Adaptation of the mouse neutropenic thigh model assay for evaluation of candidate therapeutics against clinically relevant, highly antibiotic resistant strains of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Enterococcus faecalis, Enterobacter aerogenes, Klebsiella pneumoniae.
  • Development of protective sheep monoclonal antibodies against Botulinum Neurotoxin.
  • Development of a mouse monoclonal antibody reagent panel against Botulinum Neurotoxin toxinotypes A1, A2, B, E.
  • Characterization and evaluation of anti-Stx human monoclonal antibodies for the prevention of Hemolytic Uremic Syndrome (HUS).
  • Identification of the structural components that define protective antibodies against Cryptococcus neoformans.

Research and Clinical Interests

  • Animal Model Development
  • In vivo Evaluation of Candidate Therapeutics
  • Development of Antibody-Based Therapeutics
  • Infection Control

Teaching

Veterinary Medicine Curriculum:

Microbial Pathogenesis

Emerging & Exotic Diseases of Animals & Initial Accreditation Training

General Parasitology

Basic Pharmacology

Immunology

 

Conservation Medicine Curriculum:

Field & Laboratory Techniques

 

Infectious Diseases & Global Health (MS-IDGH) Curriculum:

Infectious Diseases of Humans and Animals I

Microbiology and Immunology Techniques

Infectious Diseases of Humans and Animals II

Research Interests by Area

Infectious Diseases: Prevention and Control
  • Botulism antidotes and anti-toxins

Laboratory Personnel

  • Karen Baldwin, Animal Specialist
  • Courtney Boucher, Research Technician
  • Kwasi Ofori, Research Assistant