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Donna Akiyoshi - BA, BS, MBA, PhD

Donna Akiyoshi
Research Associate Professor
Assistant Director, RBL
Molecular Genetics, Microbial Pathogenesis

Campus Phone:



  • MBA - Worcester Polytechnic Institute - 1997
  • PhD Biochemistry/Biophysics - Oregon State University - 1983
  • BS Biological Sciences/Chemistry - University of California at Irvine - 1977
  1. Widmer, G., Dilo, J., Tumwine, J.K., Tzipori, S., Akiyoshi, D.E. 2013. Frequent occurrence of mixed enterocytozoon bieneusi infections in humans. Applied and Environmental Microbiology.
  2. Chappell, C.L., Okhuysen, P.C., Langer-Curry, R.C., Akiyoshi, D.E., Widmer, G., Tzipori, S. 2011. Cryptosporidium meleagridis: Infectivity in healthy adult volunteers. American Journal of Tropical Medicine and Hygiene.
  3. Keeling, P.J., Corradi, N., Morrison, H.G., Haag, K.L., Ebert, D., Weiss, L.M., Akiyoshi, D.E., Tzipori, S. 2010. The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism. Genome Biology and Evolution.
  4. Akiyoshi, D.E., Sheoran, A.S., Rich, C.M., Richard, L., Chapman-Bonofiglio, S., Tzipori, S. 2010. Evaluation of Fab and F(ab′)2fragments and isotype variants of a recombinant human monoclonal antibody against Shiga toxin 2. Infection and Immunity.
  5. Widmer, G., Akiyoshi, D.E. 2010. Host-specific segregation of ribosomal nucleotide sequence diversity in the microsporidian Enterocytozoon bieneusi. Infection, Genetics and Evolution.
  6. Alvarado, J.J., Nemkal, A., Sauder, J.M., Russell, M., Akiyoshi, D.E., Shi, W., Almo, S.C., Weiss, L.M. 2009. Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470. Molecular and Biochemical Parasitology.
  7. Akiyoshi, D.E., Morrison, H.G., Lei, S., Feng, X., Zhang, Q., Corradi, N., Mayanja, H., Tumwine, J.K., Keeling, P.J., Weiss, L.M., Tzipori, S. 2009. Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. PLoS Pathogens.
  8. Tanriverdi, S., Grinberg, A., Chalmers, R.M., Hunter, P.R., Petrovic, Z., Akiyoshi, D.E., London, E., Zhang, L., Tzipori, S., Tumwine, J.K., Widmer, G. 2008. Inferences about the global population structures of Cryptosporidium parvum and Cryptosporidium hominis. Applied and Environmental Microbiology.
  9. Corradi, N., Akiyoshi, D.E., Morrison, H.G., Feng, X., Weiss, L.M., Tzipori, S., Keeling, P.J. 2007. Patterns of genome evolution among the Microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and enterocytozoon bieneusi. PLoS ONE.
  10. Feng, X., Akiyoshi, D.E., Widmer, G., Tzipori, S. 2007. Characterization of subtilase protease in Cryptosporidium parvum and C. hominis. Journal of Parasitology.
  11. Akiyoshi, D.E., Weiss, L.M., Feng, X., Williams, B.A.P., Keeling, P.J., Zhang, Q., Tzipori, S. 2007. Analysis of the β-tubulin genes from Enterocytozoon bieneusi isolates from a human and rhesus macaque. Journal of Eukaryotic Microbiology.
  12. Chappell, C.L., Okhuysen, P.C., Langer-Curry, R., Widmer, G., Akiyoshi, D.E., Tanriverdi, S., Tzipori, S. 2006. Cryptosporidium hominis: Experimental challenge of healthy adults. American Journal of Tropical Medicine and Hygiene.
  13. Akiyoshi, D.E., Tumwine, J.K., Bakeera-Kitaka, S., Tzipori, S. 2006. Subtype analysis of Cryptosporidium isolates from children in Uganda. Journal of Parasitology.
  14. Feng, X., Akiyoshi, D.E., Sheoran, A., Singh, I., Hanawalt, J., Zhang, Q., Widmer, G., Tzipori, S. 2006. Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents. Infection and Immunity.
  15. Girouard, D., Gallant, J., Akiyoshi, D.E., Nunnari, J., Tzipori, S. 2006. Failure to propagate Cryptosporidium spp. in cell-free culture. Journal of Parasitology.
  16. Tumwine, J.K., Kekitiinwa, A., Bakeera-Kitaka, S., Ndeezi, G., Downing, R., Feng, X., Akiyoshi, D.E., Tzipori, S. 2005. Cryptosporidiosis and microsporidiosis in Ugandan children with persistent diarrhea with and without concurrent infection with the human immunodeficiency virus. American Journal of Tropical Medicine and Hygiene.
  17. Akiyoshi, D.E., Rich, C.M., O'Sullivan-Murphy, S., Richard, L., Dilo, J., Donohue-Rolfe, A., Sheoran, A.S., Chapman-Bonofiglio, S., Tzipori, S. 2005. Characterization of a human monoclonal antibody against Shiga toxin 2 expressed in Chinese hamster ovary cells. Infection and Immunity.
  18. Xu, P., Widmer, G., Wang, Y., Ozaki, L.S., Alves, J.M., Serrano, M.G., Puiu, D., Manque, P., Akiyoshi, D., Mackey, A.J., Pearson, W.R., Dear, P.H., Bankier, A.T., Peterson, D.L., Abrahamsen, M.S., Kapur, V., Tzipori, S., Buck, G.A. 2004. Erratum: The genome of Cryptosporidium hominis (Nature (2004) 431 (1107-1112)). Nature.
  19. Xu, P., Widmer, G., Wang, Y., Ozaki, L.S., Alves, J.M., Serrano, M.G., Pulu, D., Manque, P., Akiyoshi, D., Mackey, A.J., Pearson, W.R., Dear, P.H., Bankler, A.T., Peterson, D.L., Abrahamsen, M.S., Kapur, V., Tzipori, S., Buck, G.A. 2004. The genome of Cryptosporidium hominis. Nature.
  20. Tzipori, S., Sheoran, A., Akiyoshi, D., Donohue-Rolfe, A., Trachtman, H. 2004. Antibody therapy in the management of Shiga toxin-induced hemolytic uremic syndrome. Clinical Microbiology Reviews.

General Research Interests

  • Molecular biology related research including gene expression, molecular genetics, genome sequencing, two-hybrid systems and phage display.

Selected Research Projects

  • Expression of recombinant human antibodies against E. coli O157:H7 Shiga toxin 1 & 2 in CHO cells
  • Protozoan research in the areas of Cryptosporidium and microsporidia

Research Interests by Area

Infectious Diseases: Genetics and Genomics
  • Cryptosporidium genomics