Cummings School of Veterinary Medicine at Tufts University
Donna Akiyoshi

Research Associate Professor
Assistant Director, RBL

Molecular Genetics, Microbial Pathogenesis

Campus Phone:
508-839-7935

Fax:
508-839-7911

Education

  • MBA - Worcester Polytechnic Institute - 1997
  • PhD Biochemistry/Biophysics - Oregon State University - 1983
  • BS Biological Sciences/Chemistry - University of California at Irvine - 1977

Frequent occurrence of mixed enterocytozoon bieneusi infections in humans

Author(s):Widmer, G. | Dilo, J. | Tumwine, J.K. | Tzipori, S. | Akiyoshi, D.E.
Publication year: 2013
Journal / Book title: Applied and Environmental Microbiology

Cryptosporidium meleagridis: Infectivity in healthy adult volunteers

Author(s):Chappell, C.L. | Okhuysen, P.C. | Langer-Curry, R.C. | Akiyoshi, D.E. | Widmer, G. | Tzipori, S.
Publication year: 2011
Journal / Book title: American Journal of Tropical Medicine and Hygiene

The reduced genome of the parasitic microsporidian Enterocytozoon bieneusi lacks genes for core carbon metabolism

Author(s):Keeling, P.J. | Corradi, N. | Morrison, H.G. | Haag, K.L. | Ebert, D. | Weiss, L.M. | Akiyoshi, D.E. | Tzipori, S.
Publication year: 2010
Journal / Book title: Genome Biology and Evolution

Evaluation of Fab and F(ab′)2 fragments and isotype variants of a recombinant human monoclonal antibody against Shiga toxin 2

Author(s):Akiyoshi, D.E. | Sheoran, A.S. | Rich, C.M. | Richard, L. | Chapman-Bonofiglio, S. | Tzipori, S.
Publication year: 2010
Journal / Book title: Infection and Immunity

Host-specific segregation of ribosomal nucleotide sequence diversity in the microsporidian Enterocytozoon bieneusi

Author(s):Widmer, G. | Akiyoshi, D.E.
Publication year: 2010
Journal / Book title: Infection, Genetics and Evolution

OpuC - An ABC transporter that is associated with Staphylococcus aureus pathogenesis

Author(s):Kiran, M.D. | Akiyoshi, D.E. | Giacometti, A. | Cirioni, O. | Scalise, G. | Balaban, N.
Publication year: 2009
Journal / Book title: International Journal of Artificial Organs

Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470

Author(s):Alvarado, J.J. | Nemkal, A. | Sauder, J.M. | Russell, M. | Akiyoshi, D.E. | Shi, W. | Almo, S.C. | Weiss, L.M.
Publication year: 2009
Journal / Book title: Molecular and Biochemical Parasitology

Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi

Author(s):Akiyoshi, D.E. | Morrison, H.G. | Lei, S. | Feng, X. | Zhang, Q. | Corradi, N. | Mayanja, H. | Tumwine, J.K. | Keeling, P.J. | Weiss, L.M. | Tzipori, S.
Publication year: 2009
Journal / Book title: PLoS Pathogens

Inferences about the global population structures of Cryptosporidium parvum and Cryptosporidium hominis

Author(s):Tanriverdi, S. | Grinberg, A. | Chalmers, R.M. | Hunter, P.R. | Petrovic, Z. | Akiyoshi, D.E. | London, E. | Zhang, L. | Tzipori, S. | Tumwine, J.K. | Widmer, G.
Publication year: 2008
Journal / Book title: Applied and Environmental Microbiology

Patterns of genome evolution among the Microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and enterocytozoon bieneusi

Author(s):Corradi, N. | Akiyoshi, D.E. | Morrison, H.G. | Feng, X. | Weiss, L.M. | Tzipori, S. | Keeling, P.J.
Publication year: 2007
Journal / Book title: PLoS ONE

Characterization of subtilase protease in Cryptosporidium parvum and C. hominis

Author(s):Feng, X. | Akiyoshi, D.E. | Widmer, G. | Tzipori, S.
Publication year: 2007
Journal / Book title: Journal of Parasitology

Analysis of the β-tubulin genes from Enterocytozoon bieneusi isolates from a human and rhesus macaque

Author(s):Akiyoshi, D.E. | Weiss, L.M. | Feng, X. | Williams, B.A.P. | Keeling, P.J. | Zhang, Q. | Tzipori, S.
Publication year: 2007
Journal / Book title: Journal of Eukaryotic Microbiology

Cryptosporidium hominis: Experimental challenge of healthy adults

Author(s):Chappell, C.L. | Okhuysen, P.C. | Langer-Curry, R. | Widmer, G. | Akiyoshi, D.E. | Tanriverdi, S. | Tzipori, S.
Publication year: 2006
Journal / Book title: American Journal of Tropical Medicine and Hygiene

Subtype analysis of Cryptosporidium isolates from children in Uganda

Author(s):Akiyoshi, D.E. | Tumwine, J.K. | Bakeera-Kitaka, S. | Tzipori, S.
Publication year: 2006
Journal / Book title: Journal of Parasitology

Serial propagation of the microsporidian Enterocytozoon bieneusi of human origin in immunocompromised rodents

Author(s):Feng, X. | Akiyoshi, D.E. | Sheoran, A. | Singh, I. | Hanawalt, J. | Zhang, Q. | Widmer, G. | Tzipori, S.
Publication year: 2006
Journal / Book title: Infection and Immunity

Failure to propagate Cryptosporidium spp. in cell-free culture

Author(s):Girouard, D. | Gallant, J. | Akiyoshi, D.E. | Nunnari, J. | Tzipori, S.
Publication year: 2006
Journal / Book title: Journal of Parasitology

Cryptosporidiosis and microsporidiosis in Ugandan children with persistent diarrhea with and without concurrent infection with the human immunodeficiency virus

Author(s):Tumwine, J.K. | Kekitiinwa, A. | Bakeera-Kitaka, S. | Ndeezi, G. | Downing, R. | Feng, X. | Akiyoshi, D.E. | Tzipori, S.
Publication year: 2005
Journal / Book title: American Journal of Tropical Medicine and Hygiene

Characterization of a human monoclonal antibody against Shiga toxin 2 expressed in Chinese hamster ovary cells

Author(s):Akiyoshi, D.E. | Rich, C.M. | O'Sullivan-Murphy, S. | Richard, L. | Dilo, J. | Donohue-Rolfe, A. | Sheoran, A.S. | Chapman-Bonofiglio, S. | Tzipori, S.
Publication year: 2005
Journal / Book title: Infection and Immunity

Erratum: The genome of Cryptosporidium hominis (Nature (2004) 431 (1107-1112))

Author(s):Xu, P. | Widmer, G. | Wang, Y. | Ozaki, L.S. | Alves, J.M. | Serrano, M.G. | Puiu, D. | Manque, P. | Akiyoshi, D. | Mackey, A.J. | Pearson, W.R. | Dear, P.H. | Bankier, A.T. | Peterson, D.L. | Abrahamsen, M.S. | Kapur, V. | Tzipori, S. | Buck, G.A.
Publication year: 2004
Journal / Book title: Nature

The genome of Cryptosporidium hominis

Author(s):Xu, P. | Widmer, G. | Wang, Y. | Ozaki, L.S. | Alves, J.M. | Serrano, M.G. | Pulu, D. | Manque, P. | Akiyoshi, D. | Mackey, A.J. | Pearson, W.R. | Dear, P.H. | Bankler, A.T. | Peterson, D.L. | Abrahamsen, M.S. | Kapur, V. | Tzipori, S. | Buck, G.A.
Publication year: 2004
Journal / Book title: Nature

General Research Interests

  • Molecular biology related research including gene expression, molecular genetics, genome sequencing, two-hybrid systems and phage display.

Selected Research Projects

  • Expression of recombinant human antibodies against E. coli O157:H7 Shiga toxin 1 & 2 in CHO cells
  • Protozoan research in the areas of Cryptosporidium and microsporidia

Research Interests by Area

Infectious Diseases: Genetics and Genomics
  • Cryptosporidium genomics