Cummings School of Veterinary Medicine at Tufts University
Arthur Donohue-Rolfe

Associate Professor & Chair

Infectious Disease, Microbial Pathogenesis
Infectious Diseases

Campus Phone:
508-839-7919

Fax:
508-839-8787

Education

  • PhD - Tufts University - 1979
  • BS - Haverford College - 1971

Allele- and Tir-independent functions of intimin in diverse animal infection models

Author(s):Mallick, E.M. | Brady, M.J. | Luperchio, S.A. | Vanguri, V.K. | Magoun, L. | Liu, H. | Sheppard, B.J. | Mukherjee, J. | Donohue-Rolfe, A. | Tzipori, S. | Leong, J.M. | Schauer, D.B.
Publication year: 2012
Journal / Book title: Frontiers in Microbiology

Enhanced actin pedestal formation by enterohemorrhagic Escherichia coli O157:H7 adapted to the mammalian host

Author(s):Brady, M.J. | Radhakrishnan, P. | Liu, H. | Magoun, L. | Murphy, K.C. | Mukherjee, J. | Donohue-Rolfe, A. | Tzipori, S. | Leong, J.M.
Publication year: 2011
Journal / Book title: Frontiers in Microbiology

In vivo versus in vitro protein abundance analysis of Shigella dysenteriae type 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism

Author(s):Kuntumalla, S. | Zhang, Q. | Braisted, J.C. | Fleischmann, R.D. | Peterson, S.N. | Donohue-Rolfe, A. | Tzipori, S. | Pieper, R.
Publication year: 2011
Journal / Book title: BMC Microbiology

The Shigella dysenteriae serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins

Author(s):Pieper, R. | Zhang, Q. | Parmar, P.P. | Huang, S.-T. | Clark, D.J. | Alami, H. | Donohue-Rolfe, A. | Fleischmann, R.D. | Peterson, S.N. | Tzipori, S.
Publication year: 2009
Journal / Book title: Proteomics

Comparison of two label-free global quantitation methods, APEX and 2D gel electrophoresis, applied to the Shigella dysenteriae proteome

Author(s):Kuntumalla, S. | Braisted, J.C. | Huang, S.-T. | Parmar, P.P. | Clark, D.J. | Alami, H. | Zhang, Q. | Donohue-Rolfe, A. | Tzipori, S. | Fleischmann, R.D. | Peterson, S.N. | Pieper, R.
Publication year: 2009
Journal / Book title: Proteome Science

Gnotobiotic piglet infection model for evaluating the safe use of antibiotics against Escherichia coli O157:H7 infection

Author(s):Zhang, Q. | Donohue-Rolfe, A. | Krautz-Peterson, G. | Sevo, M. | Parry, N. | Abeijon, C. | Tzipori, S.
Publication year: 2009
Journal / Book title: Journal of Infectious Diseases

EspF U, a type III-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by E. coli O157:H7

Author(s):Ritchie, J.M. | Brady, M.J. | Riley, K.N. | Ho, T.D. | Campellone, K.G. | Herman, I.M. | Donohue-Rolfe, A. | Tzipori, S. | Waldor, M.K. | Leong, J.M.
Publication year: 2008
Journal / Book title: Cellular Microbiology

Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7

Author(s):Campellone, K.G. | Roe, A.J. | Løbner-Olesen, A. | Murphy, K.C. | Magoun, L. | Brady, M.J. | Donohue-Rolfe, A. | Tzipori, S. | Gally, D.L. | Leong, J.M. | Marinus, M.G.
Publication year: 2007
Journal / Book title: Molecular Microbiology

Human antibody against Shiga toxin 2 administered to piglets after the onset of diarrhea due to Escherichia coli O157:H7 prevents fatal systemic complications

Author(s):Sheoran, A.S. | Chapman-Bonofiglio, S. | Harvey, B.R. | Mukherjee, J. | Georgiou, G. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2005
Journal / Book title: Infection and Immunity

Characterization of a human monoclonal antibody against Shiga toxin 2 expressed in Chinese hamster ovary cells

Author(s):Akiyoshi, D.E. | Rich, C.M. | O'Sullivan-Murphy, S. | Richard, L. | Dilo, J. | Donohue-Rolfe, A. | Sheoran, A.S. | Chapman-Bonofiglio, S. | Tzipori, S.
Publication year: 2005
Journal / Book title: Infection and Immunity

Antibody therapy in the management of Shiga toxin-induced hemolytic uremic syndrome

Author(s):Tzipori, S. | Sheoran, A. | Akiyoshi, D. | Donohue-Rolfe, A. | Trachtman, H.
Publication year: 2004
Journal / Book title: Clinical Microbiology Reviews

Stx2-specific human monoclonal antibodies protect mice against lethal infection with Escherichia coli expressing Stx2 variants

Author(s):Sheoran, A.S. | Chapman, S. | Singh, P. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2003
Journal / Book title: Infection and Immunity

Production and characterization of protective human antibodies against Shiga toxin 1

Author(s):Mukherjee, J. | Chios, K. | Fishwild, D. | Hudson, D. | O'Donnell, S. | Rich, S.M. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2002
Journal / Book title: Infection and Immunity

Human Stx2-specific monoclonal antibodies prevent systemic complications of Escherichia coli O157:H7 infection

Author(s):Mukherjee, J. | Chios, K. | Fishwild, D. | Hudson, D. | O'Donnell, S. | Rich, S.M. | Donohue-Rolfe, A. | Tzipori, S.
Publication year: 2002
Journal / Book title: Infection and Immunity

Localization of the binding site for the oligosaccharide moiety of Gb3 on verotoxin 1 using NMR residual dipolar coupling measurements

Author(s):Thompson, G.S. | Shimizu, H. | Homans, S.W. | Donohue-Rolfe, A.
Publication year: 2000
Journal / Book title: Biochemistry

Escherichia coli 0157:H7 strains that express Shiga toxin (Stx) 2 alone are more neurotropic for gnotobiotic piglets than are isotypes producing only Stx1 or both Stx1 and Stx2

Author(s):Donohue-Rolfe, A. | Kondova, I. | Oswald, S. | Hutto, D. | Tzipori, S.
Publication year: 2000
Journal / Book title: Journal of Infectious Diseases

Syntheses and immunologic properties of Escherichia coli O157 O-specific polysaccharide and Shiga toxin 1 B subunit conjugates in mice

Author(s):Konadu, E. | Donohue-Rolfe, A. | Calderwood, S.B. | Pozsgay, V. | Shiloach, J. | Robbins, J.B. | Szu, S.C.
Publication year: 1999
Journal / Book title: Infection and Immunity

Antibody-based protection of gnotobiotic piglets infected with Escherichia coli O157:H7 against systemic complications associated with Shiga toxin 2

Author(s):Donohue-Rolfe, A. | Kondova, I. | Mukherjee, J. | Chios, K. | Hutto, D. | Tzipori, S.
Publication year: 1999
Journal / Book title: Infection and Immunity

Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens

Author(s):Fuchs, S. | Mühldorfer, I. | Donohue-Rolfe, A. | Kerényi, M. | Emödy, L. | Alexiev, R. | Nenkov, P. | Hacker, J.
Publication year: 1999
Journal / Book title: Microbial Pathogenesis

Derivation of the bound-state conformation of a ligand in a weakly aligned ligand - Protein complex

Author(s):Shimizu, H. | Donohue-Rolfe, A. | Homans, S.W.
Publication year: 1999
Journal / Book title: Journal of the American Chemical Society

General Research Interests

My general research interest is the pathogenesis of enteric bacterial pathogens. My particular current interest is understanding the pathogenesis of enterohemorrhagic E. coli (EHEC). EHEC produces two toxins, Shiga toxin 1 and Shiga toxin 2. My laboratory has studied the basic structure of these toxins and is continuing to study their role in disease and the effects of immune therapy directed against the toxins may have in ameliorating the disease.

Selected Research Projects

  • Antibiotic treatment of EHEC infections. The treatment with antibiotics of human patients infected with EHEC is controversial. Studies have shown that treatment with some antibiotics may, in fact, worsen the clinical outcome. This study investigates the mechanisms by which certain antibiotics lead to a more severe illness. Selection of antibiotics which actually improve the clinical outcome will be a goal of this project.
  • Shiga toxin 2 and its association with hemolytic uremic syndrome (HUS) HUS development is a systemic complication of EHEC infections and is strongly associated with EHEC strains that produce just Shiga toxin 2. HUS produces not only temporary kidney malfunctioning but can also lead to permanent kidney malfunctioning and even death. We are studying why Shiga toxin 2 is more associated with HUS development than Shiga toxin 1.

Research Interests by Area

Infectious Diseases: Pathogenesis/Host Response
  • E. Coli O157 toxins
Infectious Diseases: Prevention and Control
  • Antibiotic treatment of EHEC infections

Major Specialized Equipment

  • ELISA plate reader
  • Electroporator