Dr. Andrew Hoffman is a Professor of Large Animal Internal Medicine with three decades of experience teaching, clinical service, and research. Dr. Hoffman’s major areas of expertise include pulmonary physiology, tissue engineering, and stem cell-based therapies. Dr. Hoffman developed the first outpatient pulmonary function testing unit for veterinary patients in North America which currently operates in the Hospital for Large Animals. He directs the Regenerative Medicine Laboratory (RML) that serves as a research and laboratory core for Clinical Science faculty engaged in research related to stem cells and regenerative medicine. Dr. Hoffman’s laboratory has continuously received funding from the National Institute of Health (NIH) since 1999, in addition to numerous industry and foundation awards.
At present, the main focus of Dr. Hoffman’s clinical research is mesenchymal stem cell (MSC) safety, potency, and biological activity, in veterinary and by extension human patients with a variety of persistent diseases. Mechanisms by which MSCs convey immunoregulatory and anti-inflammatory signals to recipient cells that are under investigation include, the release of soluble mediators and extracellular vesicles (nanoparticles). In addition to these more basic science questions, the RML directed by Dr. Hoffman facilitates clinical trials in veterinary patients, including the training and supervision of clinical trial technicians and coordinators, laboratory support including measurement of biomarkers and cells (flow cytometry), study design, quality control, and biostatistics. Examples of veterinary clinical trials facilitated by the RML include the effects of MSCs on autoimmune disease (perianal fistulas, granulomatous meningoencephalomyelitis, immune complex glomerulonephritis, and hepatitis), sepsis, osteoarthritis, and inter-vertebral disc disease progression in dogs. Additionally Dr. Hoffman is currently a Principal Investigator on a translational project funded by NIH entitled ‘Autologous mesenchymal stem cell therapy for emphysema’ (R01 HL112987-01A1) which aims to understand how stem cells enhanced by biological scaffolds can activate the repair of the emphysema lung.
The RML also offers Post-Doctoral training opportunities for veterinarians who have completed their Veterinary Residency programs, which are supported by NIH and Foundation funding. Finally, the RML is responsible for maintaining the animal stem cell bank, comprised of a variety of stem cell lines from veterinary patients with naturally occurring diseases for studies of disease mechanisms, and cell lines from normal animals (dogs, cats, horses) intended for use in clinical trials.. Dr. Andrew Hoffman received an Exceptional Service Award at Tufts University Cummings School of Veterinary Medicine in 2013 for activities as Director of the RML. He resides with his family in Sutton, MA.
- D.V.Sc. - University of Guelph, Ontario Veterinary College - 1990
- D.V.M. - Cornell University - 1985
- ACVIM (Large Animal Medicine)
- Yang, V.K., Tai, A.K., Huh, T.P., Meola, D.M., Juhr, C.M., Robinson, N.A., Hoffman, A.M. 2018. Dysregulation of valvular interstitial cell let-7c, MIR-17, MIR-20a, and MIR-30d in naturally occurring canine myxomatous mitral valve disease. PLoS ONE.
- Borel, F., Sun, H., Zieger, M., Cox, A., Cardozo, B., Li, W., Oliveira, G., Davis, A., Gruntman, A., Flotte, T.R., Brodsky, M.H., Hoffman, A.M., Elmallah, M.K., Mueller, C. 2018. Editing out five Serpina1 paralogs to create a mouse model of genetic emphysema. Proceedings of the National Academy of Sciences of the United States of America.
- Clayton, A., Buschmann, D., Byrd, J.B., Carter, D.R.F., Cheng, L., Compton, C., Daaboul, G., Devitt, A., Falcon-Perez, J.M., Gardiner, C., Gustafson, D., Harrison, P., Helmbrecht, C., Hendrix, A., Hill, A., Hoffman, A., Jones, J.C., Kalluri, R., Kang, J.Y., Kirchner, B., Lässer, C., Lawson, C., Lenassi, M., Levin, C., Llorente, A., Martens-Uzunova, E.S., Möller, A., Musante, L., Ochiya, T., Pink, R.C., Tahara, H., Wauben, M.H.M., Webber, J.P., Welsh, J.A., Witwer, K.W., Yin, H., Nieuwland, R. 2018. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017. Journal of Extracellular Vesicles.
- Yang, V.K., Loughran, K.A., Meola, D.M., Juhr, C.M., Thane, K.E., Davis, A.M., Hoffman, A.M. 2017. Circulating exosome microRNA associated with heart failure secondary to myxomatous mitral valve disease in a naturally occurring canine model. Journal of Extracellular Vesicles.
- Davis, A.M., Thane, K.E., Hoffman, A.M. 2017. Practical methods for assessing emphysema severity based on estimation of linear mean intercept (Lm) in the context of animal models of alpha-1 antitrypsin deficiency. Methods in Molecular Biology.
- Platz, J., Bonenfant, N.R., Uhl, F.E., Coffey, A.L., McKnight, T., Parsons, C., Sokocevic, D., Borg, Z.D., Lam, Y.-W., Deng, B., Fields, J.G., Desarno, M., Loi, R., Hoffman, A.M., Bianchi, J., Dacken, B., Petersen, T., Wagner, D.E., Weiss, D.J. 2016. Comparative Decellularization and Recellularization of Wild-Type and Alpha 1,3 Galactosyltransferase Knockout Pig Lungs: A Model for Ex Vivo Xenogeneic Lung Bioengineering and Transplantation. Tissue Engineering - Part C: Methods.
- Hoffman, A.M., Dow, S.W. 2016. Concise Review: Stem Cell Trials Using Companion Animal Disease Models. Stem Cells.
- Wichtel, M., Gomez, D., Burton, S., Wichtel, J., Hoffman, A. 2016. Relationships between equine airway reactivity measured byÂ flowmetric plethysmography and specific indicators of airway inflammation in horses with suspected inflammatory airway disease. Equine veterinary journal.
- Ferrer, L., Kimbrel, E.A., Lam, A., Falk, E.B., Zewe, C., Juopperi, T., Lanza, R., Hoffman, A. 2016. Treatment of perianal fistulas with human embryonic stem cell-derived mesenchymal stem cells: A canine model of human fistulizing Crohn's disease. Regenerative Medicine.
- Houtsma, A., Bedenice, D., Pusterla, N., Pugliese, B., Mapes, S., Hoffman, A.M., Paxson, J., Rozanski, E., Mukherjee, J., Wigley, M., Mazan, M.R. 2015. Association between inflammatory airway disease of horses and exposure to respiratory viruses: A case control study. Multidisciplinary Respiratory Medicine.
- Cruz, F.F., Borg, Z.D., Goodwin, M., Sokocevic, D., Wagner, D.E., Coffey, A., Antunes, M., Robinson, K.L., Mitsialis, S.A., Kourembanas, S., Thane, K., Hoffman, A.M., McKenna, D.H., Rocco, P.R.M., Weiss, D.J. 2015. Systemic administration of human bone marrow-derived mesenchymal stromal cell extracellular vesicles ameliorates Aspergillus hyphal extract-induced allergic airway inflammation in immunocompetent mice. Stem Cells Translational Medicine.
- Graham, J.E., Meola, D.M., Kini, N.R., Hoffman, A.M. 2015. Comparison of the effects of glycerol, dimethyl sulfoxide, and hydroxyethyl starch solutions for cryopreservation of avian red blood cells. American Journal of Veterinary Research.
- Rettmer, H., Hoffman, A.M., Lanz, S., Oertly, M., Gerber, V. 2015. Owner-reported coughing and nasal discharge are associated with clinical findings, arterial oxygen tension, mucus score and bronchoprovocation in horses with recurrent airway obstruction in a field setting. Equine Veterinary Journal.
- Bellas, E., Rollins, A., Moreau, J.E., Lo, T., Quinn, K.P., Fourligas, N., Georgakoudi, I., Leisk, G.G., Mazan, M., Thane, K.E., Taeymans, O., Hoffman, A.M., Kaplan, D.L., Kirker-Head, C.A. 2015. Equine model for soft-tissue regeneration. Journal of Biomedical Materials Research - Part B Applied Biomaterials.
- Pacheco, A.P., Paradis, M.R., Hoffman, A.M., Hermida, P., Sanchez, A., Nadeau, J.A., Tufts, M., Mazan, M.R. 2014. Age effects on blood gas, spirometry, airway reactivity, and bronchoalveolar lavage fluid cytology in clinically healthy horses. Journal of Veterinary Internal Medicine.
- Thane, K., Ingenito, E.P., Hoffman, A.M. 2014. Lung regeneration and translational implications of the postpneumonectomy model. Translational Research.
- Mazan, M.R., Lascola, K., Bruns, S.J., Hoffman, A.M. 2014. Use of a novel one-nostril mask-spacer device to evaluate airway hyperresponsiveness (AHR) in horses after chronic administration of albuterol. Canadian Journal of Veterinary Research.
- Paxson, J.A., Gruntman, A.M., Davis, A.M., Parkin, C.M., Ingenito, E.P., Hoffman, A.M. 2013. Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy. Stem Cells and Development.
- Wagner, D.E., Bonvillain, R.W., Jensen, T., Girard, E.D., Bunnell, B.A., Finck, C.M., Hoffman, A.M., Weiss, D.J. 2013. Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds. Respirology.
- Matthay, M.A., Anversa, P., Bhattacharya, J., Burnett, B.K., Chapman, H.A., Hare, J.M., Hei, D.J., Hoffman, A.M., Kourembanas, S., McKenna, D.H., Ortiz, L.A., Ott, H.C., Tente, W., Thebaud, B., Trapnell, B.C., Weiss, D.J., Yuan, J.X.-J., Blaisdell, C.J. 2013. Cell therapy for lung diseases report from an NIH-NHLBI workshop, november 13-14, 2012. American Journal of Respiratory and Critical Care Medicine.
Research and Clinical Interests
- Pulmonary Physiology
- Cellular and Molecular Biology
- Regenerative Medicine
Stem cells and organ regeneration
The cellular and molecular basis for organ regeneration with emphasis on lung; role of stem / progenitor cells in lung regeneration; molecular basis for self-renewal and differentiation in lung stem cells; growth signaling pathways initiated by mesenchymal stem cells in the lung (LMSCs.
Veterinary and translational research
Devices for pulmonary function testing; diagnosis of inflammatory airway disease using bronchoalveolar cytology; aerosol delivery devices; respiratory physiology of marine mammals; non-surgical lung volume reduction; cell-based therapies.
Research Interests by Area
- Stem cell biology. Tissue regeneration. Clinical applications of stem cell therapies
Spontaneous Animal Disease Models
- Regenerative medicine applications for spontaneous animal diseases
Research Interests by Organ System and Disease
- Comparative respiratory physiology,lung mechanics, device development, asthma, emphysema. Use of stem cells to regenerate lung tissue. Tissue engineering approaches to lung disease